rabbit serum (Vector Laboratories)

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Vector Laboratories rabbit serum
Rabbit Serum, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 823 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/S-5000-20/Normal+Rabbit+Serum+Blocking+Solution/10__3390_slash_jfb17100503-69-46-48
Average 94 stars, based on 823 article reviews

rabbit serum - by Bioz Stars, 2026-10

94/100 stars

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Article Title: IL-4 treatment induces apoptosis of blood monocytes and proliferation of recruited injury-associated macrophages to resolve liver injury.
Article Snippet: Non-specific binding of antibodies was blocked with Mouse on Mouse (MOM) IgG blocking reagent (Vector Laboratories) for one hour, followed by washing twice with PBS, and incubation with MOM protein concentrate (Vector Laboratories) for five minutes.

Blocking Assay:

Article Title: Modulation of macrophage polarization with acute administration of Vericiguat after myocardial infarction.
Article Snippet: 26 Vericiguat (Merck, marketed as Verquvo) is a soluble guanylate cyclase stimulator that is FDA-approved for 27 use in heart failure patients with reduced or mildly reduced ejection fraction (HFrEF, HFmrEF) to decrease 28 heart failure hospitalization and cardiovascular mortality.. We hypothesized that earlier administration of 29 vericiguat post-MI would reduce the workload of the viable cardiomyocytes, leading to an earlier switch 30 towards anti-inflammatory macrophages and reduced adverse remodeling.. Male and female C57BL/6J mice 31 (n=6/sex/group) underwent permanent occlusion of the left anterior descending coronary artery followed by 32 implantation of a subcutaneous osmotic mini-pump (vericiguat or saline) 24 hours later.

Article Title: IL-4 treatment induces apoptosis of blood monocytes and proliferation of recruited injury-associated macrophages to resolve liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Carbon Tetrachloride (CCl4) Sigma-Aldrich 289116-100ML 31996-1L EdU (5-ethynyl-2′-deoxyuridine) Thermofisher A10044 Donkey Serum Abcam ab7475 FCS Bodinco 5010 ProLong Diamond Thermo Fisher P36970 Saponin Sigma-Aldrich 4521 BSA Merck A8577-10ML Antigenfix Diapath P0014 DNase I Sigma-Aldrich 04 536 282 001 Collagenase A Sigma-Aldrich 11088793001 RPMI 1640 ThermoFisher 11875093 Collagenase V Sigma-Aldrich C9263-1G Collagenase D Roche 11 088 882 001 Dispase ThermoFisher 17105041 DNase 1 Roche 101 104 159 001 RPMI 1640 ThermoFisher 31870025 Bovine serum albumin Sigma-Aldrich A7906-100G EDTA ThermoFisher 15575038 Olive Oil Sigma-Aldrich O1514-500ML BrdU Sigma-Aldrich B5002 Dulbeccos PBS ThermoFisher 14190094 Annexin V AF647 BioLegend 640912 Recombinant mouse IL-4 Peprotech 214–14 RBC lysis buffer BioLegend 420301 RBC lysis buffer Sigma-Aldrich R7757-100ML CountBrightTM Beads ThermoFisher C36950 Mouse serum ThermoFisher 10410 Annexin V Binding buffer BioLegend 422201 Foxp3 Transcription factor staining kit eBioscience 00-5121-00 MgCl2 Sigma-Aldrich 944289-250G DMEM, high glucose, glutaMAXTM supplement ThermoFisher 10566016 Penicillin-Streptomycin ThermoFisher 15140122 FBS Biosera FB-1280/500 Recombinant CSF1 Miltenyi Biotec 130-101-706 Methanol Sigma-Aldrich 34860-1L-R Chloroform ThermoFisher 4046300025 Acetic acid Sigma-Aldrich A6283-1L Xylene Chemlab CL0024015000 Trypsin Sigma-Aldrich T7168-20tab Hydrogen Peroxide Sigma-Aldrich H1009 Borax Sigma-Aldrich 71997 Vectastain Elite ABC reagent Vector PK-7100 DAB DakoCytomation K346811-2 Avidin/Biotin blocking kit Vector Laboratories SP-2001 Normal Rabbit Serum Vector S-5000-20 Tissue-Tek OCT compound Sakura 4583 Triton X-100 Sigma-Adlrich 1003287133 (Continued on next page) 22 Cell Reports 45, 117136, April 28, 2026 .. In vivo animal studies C57BL/6JCrl and congenic CD45.1+CD45.2+ mice (B6.SJL-PtprcaPep3b/BoyJ x C57BL/6JCrl), Il4ra− /− (Il4ratm1Fbb),30 Il4raflox/flox (ll4ratm2Fbb)88 and Csf1r-eGFP (Tg(Csf1r-EGFP)1Hume)89 mice were bred and maintained under specific pathogen-free facilities at the University of Edinburgh, UK, or obtained from Charles River, UK.

Activity Assay:

Incubation:

Article Title: De novo identification of potent ingredients for proteasome activation in MT101-5 using an AI-driven approach.
Article Snippet: .. Sections were then incubated overnight at 4 ◦C with a 1:1000 dilution of polyclonal rabbit anti-tyrosine hydroxylase (TH) antibody (Cat# AB152; Merck Millipore, Burlington, MA, USA; RRID: AB_390204) or a 1:250 dilution of polyclonal goat anti-ionized calciumbinding adapter molecule 1 (Iba1) antibody (Cat# AB5076; Abcam, Cambridge, UK; RRID:AB_2224402) or a 1:500 dilution of monoclonal rat anti-dopamine transporter (DAT) antibody (Cat# MAB369; Merck Millipore, Burlington, MA, USA; RRID: AB_2190413) in 0.3 % Triton X100 (Sigma-Aldrich, St. Louis, MO, USA) and 5 % normal goat serum or 5 % normal rabbit serum (Vector Labs, Burlingame, CA, USA). .. Following incubation with a 1:250 dilution of biotinylated polyclonal goat antirabbit IgG (Cat# BA-1000; Vector Labs; RRID: AB_2313606) or rabbit anti-goat IgG (Cat# AI-5000; Vector Labs; RRID: AB_2336125) or goat anti-rat IgG (Cat# PI-9400; Vector Labs; RRID: AB_2336203) for 1 h and subsequent incubation with a 1:100 dilution of avidin-biotin complex (Vector Labs) for 1 h at room temperature, peroxidase activity was visualized using 3,3-diaminobenzidine in 0.05 M Tris-buffered saline (pH 7.6).

Recombinant:

Article Title: IL-4 treatment induces apoptosis of blood monocytes and proliferation of recruited injury-associated macrophages to resolve liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Carbon Tetrachloride (CCl4) Sigma-Aldrich 289116-100ML 31996-1L EdU (5-ethynyl-2′-deoxyuridine) Thermofisher A10044 Donkey Serum Abcam ab7475 FCS Bodinco 5010 ProLong Diamond Thermo Fisher P36970 Saponin Sigma-Aldrich 4521 BSA Merck A8577-10ML Antigenfix Diapath P0014 DNase I Sigma-Aldrich 04 536 282 001 Collagenase A Sigma-Aldrich 11088793001 RPMI 1640 ThermoFisher 11875093 Collagenase V Sigma-Aldrich C9263-1G Collagenase D Roche 11 088 882 001 Dispase ThermoFisher 17105041 DNase 1 Roche 101 104 159 001 RPMI 1640 ThermoFisher 31870025 Bovine serum albumin Sigma-Aldrich A7906-100G EDTA ThermoFisher 15575038 Olive Oil Sigma-Aldrich O1514-500ML BrdU Sigma-Aldrich B5002 Dulbeccos PBS ThermoFisher 14190094 Annexin V AF647 BioLegend 640912 Recombinant mouse IL-4 Peprotech 214–14 RBC lysis buffer BioLegend 420301 RBC lysis buffer Sigma-Aldrich R7757-100ML CountBrightTM Beads ThermoFisher C36950 Mouse serum ThermoFisher 10410 Annexin V Binding buffer BioLegend 422201 Foxp3 Transcription factor staining kit eBioscience 00-5121-00 MgCl2 Sigma-Aldrich 944289-250G DMEM, high glucose, glutaMAXTM supplement ThermoFisher 10566016 Penicillin-Streptomycin ThermoFisher 15140122 FBS Biosera FB-1280/500 Recombinant CSF1 Miltenyi Biotec 130-101-706 Methanol Sigma-Aldrich 34860-1L-R Chloroform ThermoFisher 4046300025 Acetic acid Sigma-Aldrich A6283-1L Xylene Chemlab CL0024015000 Trypsin Sigma-Aldrich T7168-20tab Hydrogen Peroxide Sigma-Aldrich H1009 Borax Sigma-Aldrich 71997 Vectastain Elite ABC reagent Vector PK-7100 DAB DakoCytomation K346811-2 Avidin/Biotin blocking kit Vector Laboratories SP-2001 Normal Rabbit Serum Vector S-5000-20 Tissue-Tek OCT compound Sakura 4583 Triton X-100 Sigma-Adlrich 1003287133 (Continued on next page) 22 Cell Reports 45, 117136, April 28, 2026 .. In vivo animal studies C57BL/6JCrl and congenic CD45.1+CD45.2+ mice (B6.SJL-PtprcaPep3b/BoyJ x C57BL/6JCrl), Il4ra− /− (Il4ratm1Fbb),30 Il4raflox/flox (ll4ratm2Fbb)88 and Csf1r-eGFP (Tg(Csf1r-EGFP)1Hume)89 mice were bred and maintained under specific pathogen-free facilities at the University of Edinburgh, UK, or obtained from Charles River, UK.

Red Blood Cell Lysis:

Article Title: IL-4 treatment induces apoptosis of blood monocytes and proliferation of recruited injury-associated macrophages to resolve liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Carbon Tetrachloride (CCl4) Sigma-Aldrich 289116-100ML 31996-1L EdU (5-ethynyl-2′-deoxyuridine) Thermofisher A10044 Donkey Serum Abcam ab7475 FCS Bodinco 5010 ProLong Diamond Thermo Fisher P36970 Saponin Sigma-Aldrich 4521 BSA Merck A8577-10ML Antigenfix Diapath P0014 DNase I Sigma-Aldrich 04 536 282 001 Collagenase A Sigma-Aldrich 11088793001 RPMI 1640 ThermoFisher 11875093 Collagenase V Sigma-Aldrich C9263-1G Collagenase D Roche 11 088 882 001 Dispase ThermoFisher 17105041 DNase 1 Roche 101 104 159 001 RPMI 1640 ThermoFisher 31870025 Bovine serum albumin Sigma-Aldrich A7906-100G EDTA ThermoFisher 15575038 Olive Oil Sigma-Aldrich O1514-500ML BrdU Sigma-Aldrich B5002 Dulbeccos PBS ThermoFisher 14190094 Annexin V AF647 BioLegend 640912 Recombinant mouse IL-4 Peprotech 214–14 RBC lysis buffer BioLegend 420301 RBC lysis buffer Sigma-Aldrich R7757-100ML CountBrightTM Beads ThermoFisher C36950 Mouse serum ThermoFisher 10410 Annexin V Binding buffer BioLegend 422201 Foxp3 Transcription factor staining kit eBioscience 00-5121-00 MgCl2 Sigma-Aldrich 944289-250G DMEM, high glucose, glutaMAXTM supplement ThermoFisher 10566016 Penicillin-Streptomycin ThermoFisher 15140122 FBS Biosera FB-1280/500 Recombinant CSF1 Miltenyi Biotec 130-101-706 Methanol Sigma-Aldrich 34860-1L-R Chloroform ThermoFisher 4046300025 Acetic acid Sigma-Aldrich A6283-1L Xylene Chemlab CL0024015000 Trypsin Sigma-Aldrich T7168-20tab Hydrogen Peroxide Sigma-Aldrich H1009 Borax Sigma-Aldrich 71997 Vectastain Elite ABC reagent Vector PK-7100 DAB DakoCytomation K346811-2 Avidin/Biotin blocking kit Vector Laboratories SP-2001 Normal Rabbit Serum Vector S-5000-20 Tissue-Tek OCT compound Sakura 4583 Triton X-100 Sigma-Adlrich 1003287133 (Continued on next page) 22 Cell Reports 45, 117136, April 28, 2026 .. In vivo animal studies C57BL/6JCrl and congenic CD45.1+CD45.2+ mice (B6.SJL-PtprcaPep3b/BoyJ x C57BL/6JCrl), Il4ra− /− (Il4ratm1Fbb),30 Il4raflox/flox (ll4ratm2Fbb)88 and Csf1r-eGFP (Tg(Csf1r-EGFP)1Hume)89 mice were bred and maintained under specific pathogen-free facilities at the University of Edinburgh, UK, or obtained from Charles River, UK.

Binding Assay:

Article Title: IL-4 treatment induces apoptosis of blood monocytes and proliferation of recruited injury-associated macrophages to resolve liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Carbon Tetrachloride (CCl4) Sigma-Aldrich 289116-100ML 31996-1L EdU (5-ethynyl-2′-deoxyuridine) Thermofisher A10044 Donkey Serum Abcam ab7475 FCS Bodinco 5010 ProLong Diamond Thermo Fisher P36970 Saponin Sigma-Aldrich 4521 BSA Merck A8577-10ML Antigenfix Diapath P0014 DNase I Sigma-Aldrich 04 536 282 001 Collagenase A Sigma-Aldrich 11088793001 RPMI 1640 ThermoFisher 11875093 Collagenase V Sigma-Aldrich C9263-1G Collagenase D Roche 11 088 882 001 Dispase ThermoFisher 17105041 DNase 1 Roche 101 104 159 001 RPMI 1640 ThermoFisher 31870025 Bovine serum albumin Sigma-Aldrich A7906-100G EDTA ThermoFisher 15575038 Olive Oil Sigma-Aldrich O1514-500ML BrdU Sigma-Aldrich B5002 Dulbeccos PBS ThermoFisher 14190094 Annexin V AF647 BioLegend 640912 Recombinant mouse IL-4 Peprotech 214–14 RBC lysis buffer BioLegend 420301 RBC lysis buffer Sigma-Aldrich R7757-100ML CountBrightTM Beads ThermoFisher C36950 Mouse serum ThermoFisher 10410 Annexin V Binding buffer BioLegend 422201 Foxp3 Transcription factor staining kit eBioscience 00-5121-00 MgCl2 Sigma-Aldrich 944289-250G DMEM, high glucose, glutaMAXTM supplement ThermoFisher 10566016 Penicillin-Streptomycin ThermoFisher 15140122 FBS Biosera FB-1280/500 Recombinant CSF1 Miltenyi Biotec 130-101-706 Methanol Sigma-Aldrich 34860-1L-R Chloroform ThermoFisher 4046300025 Acetic acid Sigma-Aldrich A6283-1L Xylene Chemlab CL0024015000 Trypsin Sigma-Aldrich T7168-20tab Hydrogen Peroxide Sigma-Aldrich H1009 Borax Sigma-Aldrich 71997 Vectastain Elite ABC reagent Vector PK-7100 DAB DakoCytomation K346811-2 Avidin/Biotin blocking kit Vector Laboratories SP-2001 Normal Rabbit Serum Vector S-5000-20 Tissue-Tek OCT compound Sakura 4583 Triton X-100 Sigma-Adlrich 1003287133 (Continued on next page) 22 Cell Reports 45, 117136, April 28, 2026 .. In vivo animal studies C57BL/6JCrl and congenic CD45.1+CD45.2+ mice (B6.SJL-PtprcaPep3b/BoyJ x C57BL/6JCrl), Il4ra− /− (Il4ratm1Fbb),30 Il4raflox/flox (ll4ratm2Fbb)88 and Csf1r-eGFP (Tg(Csf1r-EGFP)1Hume)89 mice were bred and maintained under specific pathogen-free facilities at the University of Edinburgh, UK, or obtained from Charles River, UK.

Staining:

Article Title: IL-4 treatment induces apoptosis of blood monocytes and proliferation of recruited injury-associated macrophages to resolve liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Carbon Tetrachloride (CCl4) Sigma-Aldrich 289116-100ML 31996-1L EdU (5-ethynyl-2′-deoxyuridine) Thermofisher A10044 Donkey Serum Abcam ab7475 FCS Bodinco 5010 ProLong Diamond Thermo Fisher P36970 Saponin Sigma-Aldrich 4521 BSA Merck A8577-10ML Antigenfix Diapath P0014 DNase I Sigma-Aldrich 04 536 282 001 Collagenase A Sigma-Aldrich 11088793001 RPMI 1640 ThermoFisher 11875093 Collagenase V Sigma-Aldrich C9263-1G Collagenase D Roche 11 088 882 001 Dispase ThermoFisher 17105041 DNase 1 Roche 101 104 159 001 RPMI 1640 ThermoFisher 31870025 Bovine serum albumin Sigma-Aldrich A7906-100G EDTA ThermoFisher 15575038 Olive Oil Sigma-Aldrich O1514-500ML BrdU Sigma-Aldrich B5002 Dulbeccos PBS ThermoFisher 14190094 Annexin V AF647 BioLegend 640912 Recombinant mouse IL-4 Peprotech 214–14 RBC lysis buffer BioLegend 420301 RBC lysis buffer Sigma-Aldrich R7757-100ML CountBrightTM Beads ThermoFisher C36950 Mouse serum ThermoFisher 10410 Annexin V Binding buffer BioLegend 422201 Foxp3 Transcription factor staining kit eBioscience 00-5121-00 MgCl2 Sigma-Aldrich 944289-250G DMEM, high glucose, glutaMAXTM supplement ThermoFisher 10566016 Penicillin-Streptomycin ThermoFisher 15140122 FBS Biosera FB-1280/500 Recombinant CSF1 Miltenyi Biotec 130-101-706 Methanol Sigma-Aldrich 34860-1L-R Chloroform ThermoFisher 4046300025 Acetic acid Sigma-Aldrich A6283-1L Xylene Chemlab CL0024015000 Trypsin Sigma-Aldrich T7168-20tab Hydrogen Peroxide Sigma-Aldrich H1009 Borax Sigma-Aldrich 71997 Vectastain Elite ABC reagent Vector PK-7100 DAB DakoCytomation K346811-2 Avidin/Biotin blocking kit Vector Laboratories SP-2001 Normal Rabbit Serum Vector S-5000-20 Tissue-Tek OCT compound Sakura 4583 Triton X-100 Sigma-Adlrich 1003287133 (Continued on next page) 22 Cell Reports 45, 117136, April 28, 2026 .. In vivo animal studies C57BL/6JCrl and congenic CD45.1+CD45.2+ mice (B6.SJL-PtprcaPep3b/BoyJ x C57BL/6JCrl), Il4ra− /− (Il4ratm1Fbb),30 Il4raflox/flox (ll4ratm2Fbb)88 and Csf1r-eGFP (Tg(Csf1r-EGFP)1Hume)89 mice were bred and maintained under specific pathogen-free facilities at the University of Edinburgh, UK, or obtained from Charles River, UK.

Avidin-Biotin Assay:

Article Title: IL-4 treatment induces apoptosis of blood monocytes and proliferation of recruited injury-associated macrophages to resolve liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Carbon Tetrachloride (CCl4) Sigma-Aldrich 289116-100ML 31996-1L EdU (5-ethynyl-2′-deoxyuridine) Thermofisher A10044 Donkey Serum Abcam ab7475 FCS Bodinco 5010 ProLong Diamond Thermo Fisher P36970 Saponin Sigma-Aldrich 4521 BSA Merck A8577-10ML Antigenfix Diapath P0014 DNase I Sigma-Aldrich 04 536 282 001 Collagenase A Sigma-Aldrich 11088793001 RPMI 1640 ThermoFisher 11875093 Collagenase V Sigma-Aldrich C9263-1G Collagenase D Roche 11 088 882 001 Dispase ThermoFisher 17105041 DNase 1 Roche 101 104 159 001 RPMI 1640 ThermoFisher 31870025 Bovine serum albumin Sigma-Aldrich A7906-100G EDTA ThermoFisher 15575038 Olive Oil Sigma-Aldrich O1514-500ML BrdU Sigma-Aldrich B5002 Dulbeccos PBS ThermoFisher 14190094 Annexin V AF647 BioLegend 640912 Recombinant mouse IL-4 Peprotech 214–14 RBC lysis buffer BioLegend 420301 RBC lysis buffer Sigma-Aldrich R7757-100ML CountBrightTM Beads ThermoFisher C36950 Mouse serum ThermoFisher 10410 Annexin V Binding buffer BioLegend 422201 Foxp3 Transcription factor staining kit eBioscience 00-5121-00 MgCl2 Sigma-Aldrich 944289-250G DMEM, high glucose, glutaMAXTM supplement ThermoFisher 10566016 Penicillin-Streptomycin ThermoFisher 15140122 FBS Biosera FB-1280/500 Recombinant CSF1 Miltenyi Biotec 130-101-706 Methanol Sigma-Aldrich 34860-1L-R Chloroform ThermoFisher 4046300025 Acetic acid Sigma-Aldrich A6283-1L Xylene Chemlab CL0024015000 Trypsin Sigma-Aldrich T7168-20tab Hydrogen Peroxide Sigma-Aldrich H1009 Borax Sigma-Aldrich 71997 Vectastain Elite ABC reagent Vector PK-7100 DAB DakoCytomation K346811-2 Avidin/Biotin blocking kit Vector Laboratories SP-2001 Normal Rabbit Serum Vector S-5000-20 Tissue-Tek OCT compound Sakura 4583 Triton X-100 Sigma-Adlrich 1003287133 (Continued on next page) 22 Cell Reports 45, 117136, April 28, 2026 .. In vivo animal studies C57BL/6JCrl and congenic CD45.1+CD45.2+ mice (B6.SJL-PtprcaPep3b/BoyJ x C57BL/6JCrl), Il4ra− /− (Il4ratm1Fbb),30 Il4raflox/flox (ll4ratm2Fbb)88 and Csf1r-eGFP (Tg(Csf1r-EGFP)1Hume)89 mice were bred and maintained under specific pathogen-free facilities at the University of Edinburgh, UK, or obtained from Charles River, UK.

Saline:

Article Title: Tofogliflozin attenuates liver steatosis and fibrosis in non-diabetic non-alcoholic steatohepatitis mice.
Article Snippet: Deparaffinized liver Sect. (5-μm thick) were rehydrated, then boiled in citrate buffer (Ab64214, Abcam, MA, USA) for 10 min to retrieve the antigenic sites. .. The sections were rinsed several times in phosphate-buffered saline (PBS), and subsequently blocked with PBS-diluted normal rabbit serum (#30019; Vector Laboratories, Inc., Burlingame, CA, USA) for 20 min at room temperature. .. Primary antibodies (F4/80) (ab6640, Abcam, Cambridge, UK) were then added and incubated for 30 min at room temperature.

Article Title: Tofogliflozin attenuates liver steatosis and fibrosis in non-diabetic non-alcoholic steatohepatitis mice
Article Snippet: Deparaffinized liver Sect. (5-μm thick) were rehydrated, then boiled in citrate buffer (Ab64214, Abcam, MA, USA) for 10 min to retrieve the antigenic sites. .. The sections were rinsed several times in phosphate-buffered saline (PBS), and subsequently blocked with PBS-diluted normal rabbit serum (#30019; Vector Laboratories, Inc., Burlingame, CA, USA) for 20 min at room temperature. .. Primary antibodies (F4/80) (ab6640, Abcam, Cambridge, UK) were then added and incubated for 30 min at room temperature.

Protein Binding:

Article Title: Immunohistochemical Evaluation of Non-Thermal Plasma-Treated Xenogenic Bone Graft for Enhanced Bone Regeneration in a Rabbit Calvarial Defect Model
Article Snippet: .. For antigen retrieval, the sections were incubated in citrate buffer (pH 6.0) at 95 ◦C for 30 min. Endogenous peroxidase activity was blocked with 0.3% H2O2 in PBS for 30 min, followed by blocking of nonspecific protein binding with PBS containing 10% normal horse, goat, or rabbit serum (Vector Laboratories, Newark, CA, USA) for 30 min, as appropriate. ..

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